Preliminary insights into the action of non-antibiotic pharmaceuticals in the colonization of Pseudomonas aeruginosa TGC04

Mazen Wahib Mohamed Abdelhadi; João Antônio Carvalho Bandeira; Luiz Gustavo Pragana; Victor Targino Gomes; Jéssica Vieira Dantas; José Gabriel Santos de Almeida; Rafael de Almeida Travassos; Ulrich Vasconcelos

doi:10.54038/ms.v3i2.37

Autores

Mazen Wahib Mohamed Abdelhadi Misr University for Science and Technology
João Antônio Carvalho Bandeira Universidade Federal da Paraíba
Luiz Gustavo Pragana
Victor Targino Gomes Universidade Federal da Paraíba
Jéssica Vieira Dantas Universidade Federal da Paraíba
José Gabriel Santos de Almeida Universidade Federal da Paraíba
Rafael de Almeida Travassos Universidade Federal da Paraíba https://orcid.org/0000-0001-7320-4379
Ulrich Vasconcelos Universidade Federal da Paraíba https://orcid.org/0000-0001-8289-2230

DOI:

https://doi.org/10.54038/ms.v3i2.37

Palavras-chave:

Pseudomonadas, Produtos farmacêuticos não-antibióticos, Atividade antibiofilme

Resumo

Moléculas com propriedades antimicrobianas, tais como os fármacos não-antibióticos, podem pressionar a adaptação de bactérias no ambiente. Nesse trabalho, cinco fármacos não-antibióticos mais vendidos no Brasil e no Egito em 2020 (losartan 50 mg, ácido acetilsalicílico 100 mg, diclofenaco sódico 50 mg, paracetamol 750 mg e metformina 500 mg e dipirona 100 mg/mL), foram testados contra a linhagem selvagem de Pseudomonas aeruginosa TGC04, isolada por nosso grupo e que vem sendo caracterizada ao longo dos anos. Nós avaliamos a inibição da adesão do biofilme durante 48 h, empregando o teste do cristal violeta. A exposição de P. aeruginosa TGC04 aos compostos promoveu uma redução moderada da adesão, semelhante, em todas as drogas testadas, perfazendo cerca de 60%. Isto indicou que as células também estavam moderadamente aderentes. Contudo, apesar de todos os fármacos não-antibióticos não terem produzido um efeito biocida à linhagem selvagem, foi observada uma alta atividade antibiofilme, com exceção do paracetamol.

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