Coffee breaks the journey of pseudomonads: a pause for a reflection

Clóvis Queiroz Gouveia; Vitor Targino Gomes; Rafael de Almeida Travassos; Ulrich Vasconcelos

doi:10.54038/ms.v3i2.40

Autores

Clóvis Queiroz Gouveia Universidade Federal da Paraíba, Pós-Graduação em Biotecnologia
Vitor Targino Gomes Universidade Federal da Paraíba, Pós-Graduação em Biologia Celular e Molecular https://orcid.org/0000-0002-8188-106X
Rafael de Almeida Travassos Universidade Federal da Paraíba https://orcid.org/0000-0001-7320-4379
Ulrich Vasconcelos Universidade Federal da Paraíba https://orcid.org/0000-0001-8289-2230

DOI:

https://doi.org/10.54038/ms.v3i2.40

Palavras-chave:

Pseudomonas aeruginosa, cafeína, motilidade, biofilme

Resumo

Pseudomonas aeruginosa é um patógeno crítico de acordo com a lista de bactérias prioritárias da OMS. Ele é nutricionalmente versátil e expressa diferentes fatores de virulência, que contribuem para sua resiliência a estresses ambientais, bem como sua tolerância e resistência a antimicrobianos. Isso demanda a busca de estratégias para combater o crescimento microbiano, cenário em que compostos bioativos naturais estão sendo amplamente investigados. Neste trabalho, a atividade da cafeína sobre a motilidade e adesão de biofilme a superfícies de vidro e plástico foram testadas com duas linhagens de P. aeruginosa: TGC-04 (selvagem) e ATCC 9027 (padrão). Ambas aderiram fracamente às superfícies testadas. A linhagem padrão apresentou redução na adesão ao plástico e ao vidro de 46,9 e 65,0%, respectivamente, enquanto a linhagem selvagem não foi afetada pela cafeína (1024 μg/mL). Além disso, houve inibição em dois de três tipos de ensaios de motilidade testadas. Os resultados sugerem que a origem da linhagem pode ser um fator importante na suscetibilidade de P. aeruginosa à cafeína.

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