Morfologia e Histoquímica de células do fígado de fêmeas de Steindachnerina sp. (Teleostei: Characiformes)
DOI:
https://doi.org/10.54038/ms.v3i1.32Keywords:
curimatidae, fish, hepatocyte, hematoxylin and eosin, pas, toluidine blueAbstract
Fish from Curimatidae Family are of great relevance for the ecology study of aquatic environments as they are animals of detritivorous habit, thus becoming fundamental pieces in the food chains of the aquatic systems inhabited by them. The objective of this work was to evaluate and compare morphological, histological and histochemical aspects of hepatocytes from Steindachnerina females from two different aquatic systems. Sections of 6µm thickness were obtained and stained with Hematoxylin and Eosin, Toluidine Blue, Xylidine Ponceau and Periodic Acid Schiff (PAS). The slides were mounted and analyzed under a LeicaⓇ DM750 light microscope and the results recorded with the LeicaⓇ ICC50 HD imaging system. Hepatocytes from Horta and Viveiro ponds presented spherical nuclei containing large nucleoli, both evidenced by hematoxylin and dispersed and homogeneous chromatin. The secretion present in the fish cytoplasm of Horta pond was slightly basic, when compared to the fish of Viveiro pond, which was slightly acidic. RNAs that were found are more homogeneous and uniform in the fishes cytoplasm from Viveiro pond. Higher protein accumulation was observed in the cytoplasm corresponding to females from Viveiro pond. The PAS detected the presence of glycogen in greater quantity in Horta pond fish. It can be concluded that Steindachnerina females in Viveiro pond in general presented higher weight, size and more intense cellular metabolism. It means the presence of RNAs were more homogeneous and uniform as well as a higher protein accumulation in the hepatocytes cytoplasm than in those belonging to the Horta pond.
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